Journal: Analytical Chemistry

Article Title: Advances in Surrogate Neutralization Tests for High-Throughput Screening and the Point-of-Care

doi: 10.1021/acs.analchem.5c00666

Figure Lengend Snippet: Illustration of the assay principle of (a) GenScript’s cPass sVNT kit and (b) Ahn et al.’s biotin-based sVNT. Reprinted with permission from ref . Copyright 2023 Elsevier.

Article Snippet: Ahn et al. immobilized ACE2 via His 6 -tag for improved binding of RBD ( Figure b) compared to the commercial cPass sVNT from GenScript ( Figure a).

Techniques:

Journal: Analytical Chemistry

Article Title: Advances in Surrogate Neutralization Tests for High-Throughput Screening and the Point-of-Care

doi: 10.1021/acs.analchem.5c00666

Figure Lengend Snippet: Illustration of (a) the homogeneous sVNT using Tripart NanoLuc peptide fragments and (b) the molecular model of the predicted refolding of NanoLuc (PDB 5IBO ) (green) after complementation of fragments β10 and β9 driven by the interaction between SARS-CoV-2 spike trimer (purple) and ACE2 (fawn) (PDB 7A97 ). Reprinted with permission from ref . Copyright 2022 Macmillan Publishers Ltd. The article is licensed under a Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ .

Article Snippet: Ahn et al. immobilized ACE2 via His 6 -tag for improved binding of RBD ( Figure b) compared to the commercial cPass sVNT from GenScript ( Figure a).

Techniques:

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Antibody titers from the first and second blood samples by the participants’ demographics.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Infection, Sampling

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Locally estimated scatterplot smoothing curve based on data from the first blood sample collection, illustrating the relationship of antibody levels as follows: ( A – C ) inhibition rates (%) for the surrogate virus neutralization test (sVNT) against the wild-type (WT) strain, ( D – F ) inhibition rates (%) against the Omicron variant, and ( G – I ) IgG levels. The curves are plotted against the number of days since COVID-19 infection ( A , D , G ), the last vaccination ( B , E , H ), and the last exposure to COVID-19 ( C , F , I ). The blue line represents the regression line, and the shaded gray area indicates the 95% confidence interval.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Inhibition, Virus, Neutralization, Variant Assay, Infection

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Locally estimated scatterplot smoothing curve based on data from the second blood sample collection, illustrating the relationship of antibody levels as follows: ( A – C ) inhibition rates (%) for the surrogate virus neutralization test (sVNT) against the wild-type (WT) strain, ( D – F ) inhibition rates (%) against the Omicron variant, and ( G – I ) IgG levels. The curves are plotted against the number of days since COVID-19 infection ( A , D , G ), the last vaccination ( B , E , H ), and the last exposure to COVID-19 ( C , F , I ). The blue line represents the regression line, and the shaded gray area indicates the 95% confidence interval.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Inhibition, Virus, Neutralization, Variant Assay, Infection